Thermo Scientific QuantStudio 1 - CHAPTER 5 Calibrate and Verify Instrument Performance; Calibration and Verification Schedule

Calibrate and verify instrument
performance
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Calibration and verication schedule ................................... 49
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Calibration descriptions ............................................... 50
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View the calibration status and set reminders ............................ 51
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Perform ROI/uniformity, background, and dye calibrations ................ 52
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Perform instrument verication using RNase P plates ..................... 57
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Calibrate custom dyes ................................................ 62
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Calibrate for a custom melt curve experiment ............................ 68
Calibration and verification schedule
The instrument is factory-calibrated and does not require calibration at installation. To
ensure optimal performance, perform calibrations at the recommended frequency.
Note: After instrument installation, we recommend performing instrument
verication using the provided RNase P plate.
IMPORTANT! Perform calibrations and instrument runs under the environmental
conditions that are specied in “Environmental requirements“ on page 89. Exposure
to extreme temperatures can adversely aect the instrument performance and shorten
the life span of the instrument components.
To set the calibration frequency for the instrument, touch Seings4Maintenance
and Service4Calibrations4History and Reminders4Edit4Exp interval eld.
Calibration
Recommended frequency
ROI/Uniformity
• Every two years (recommended)
• Always perform new Background and Dye calibrations after
an ROI/Uniformity calibration.
Note: Performing an ROI/Uniformity calibration invalidates all
other calibrations.
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QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide
49