Thermo Scientific QuantStudio 1 - Perform Rnase P Verification

Thaw, vortex, and centrifuge an RNase P plate
IMPORTANT! Expose the RNase P plate to room temperature for no more than
45 minutes, inclusive of thawing and preparation time.
After thawing, the RNase P plate cannot be refrozen.
1.
Remove the RNase P plate from the freezer, then thaw the plate in its packaging.
• Thaw the plate for approximately 5 minutes.
• Use the plate within 30 minutes of thawing.
2.
Conrm that the bench, vortex, and centrifuge are clean. Before use, wipe the
vortex and centrifuge using a lint-free tissue.
3.
While wearing powder-free gloves, remove the plate from its packaging.
4.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 × g for 2 minutes.
5.
Conrm that the liquid in each well is at the boom of the well and free of
bubbles. If it is not, centrifuge the plate again.
IMPORTANT! Keep the boom of the plate clean. Fluids and other contaminants
on the boom of the plate can contaminate the sample block and cause an
abnormally high background signal.
1.
In the home screen, touch Seings4Maintenance and Service4RNase P
Verication.
2.
Load the plate into the instrument.
3.
Touch Start.
4.
When the run is complete and the screen displays Verication Complete, touch
View Results to conrm the status of the run.
Calibration status Action
Passed Instrument is ready for use.
Failed See “Troubleshoot verification failure“ on page 61.
Perform RNase P
verification
Chapter 5 Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide
59