Thermo Scientific QuantStudio 1 - Decontaminate the Sample Block; Materials Required; Clean the Sample Block

Decontaminate the sample block
Perform this procedure to eliminate uorescent contaminants from the instrument
sample block. Contamination is generally evident in failed background calibrations
where one or more wells consistently exhibit abnormally high signals.
CAUTION! PHYSICAL INJURY HAZARD. Do not remove the instrument
cover. There are no components inside the instrument that you can safely
service yourself. If you suspect a problem, contact Support.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
sample block temperature can reach 100°C. Allow it to cool to room
temperature before handling.
CAUTION! Before using a cleaning or decontamination method other than
those recommended by Thermo Fisher Scientic, conrm with Thermo Fisher
Scientic that the proposed method will not damage the equipment.
• Safety glasses
• Powder-free gloves
• Tissue, lint-free
• Coon or nylon swabs and lint-free cloths
• Pipee (100-µL) with pipee tips
• Deionized water
• Ethanol, 95% solution
• Bleach, 10% solution
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
sample block temperature can reach 100°C. Allow it to cool to room
temperature before handling.
IMPORTANT!
·
Wear powder-free gloves when you perform this procedure.
·
Always use deionized water to rinse wells after cleaning with bleach or ethanol
solution.
1.
Identify the contaminated wells of the sample block (see “Identify
contamination“ on page 56).
2.
Prepare the instrument and access the sample block.
a.
Power o and unplug the instrument, then allow it to cool for 15 minutes.
b.
Pull the instrument drawer forward to expose the sample block.
Materials required
Clean the sample
block
Chapter 6 Maintain the instrument
Decontaminate the sample block
6
QuantStudio
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1 Real-Time PCR System Installation, Use, and Maintenance Guide
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