Observation Possible cause Recommended action
Forgot PIN for instrument
profile
If there is not another administrator profile on
the instrument, you must restore factory
defaults (see “Restore factory defaults“ on
page 34).
High fluorescence signal The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered
in the Method tab.
Signals that exceed the limit of
normal fluorescence may
indicate fluorescent
contaminants on the plate or
the sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate
and run the experiment again.
Identify the location of contamination on the
plate or sample block.
1. Obtain or prepare a background plate (see
page 56).
2. Follow the procedures that are described
in “Identify contamination“ on page 56.
Appendix B Troubleshooting
Networking
B
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide
85
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