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General| Brand | Applied Biosystems |
|---|---|
| Model | 3730xl |
| Category | Measuring Instruments |
| Language | English |
Introduction to the Applied Biosystems 3730xl DNA Analyzer and its capabilities.
Identification and description of the main physical components of the 3730xl DNA Analyzer.
Detailed view and identification of the internal components of the 3730xl DNA Analyzer.
Explanation of the status lights and indicators on the front panel of the instrument.
Information on consumables required and their specified usage limits for the instrument.
Details the required computer hardware and software specifications for operation.
Explanation of the scientific principles and processes behind the instrument's operation.
Overview of the 3730xl Data Collection Software 5 interface and its main functions.
Instructions on how to connect and utilize the instrument with the Thermo Fisher Connect feature.
Overview of available secondary analysis software applications for data interpretation.
Glossary of key terms and definitions used within the Data Collection Software.
Step-by-step guide illustrating the typical workflow for performing a run on the instrument.
Steps to power on and log in to the computer and instrument system.
Instructions for launching and starting the Data Collection Software.
Procedures for preparing and filling the buffer, water, and waste reservoirs.
Guide on correctly placing the prepared reservoirs into the instrument.
Detailed steps for filling the anode buffer jar with the appropriate buffer.
How to check and maintain the correct level of polymer in the instrument.
Procedure to identify and address air bubbles within the instrument's pump system.
Verifying the active spectral calibration is appropriate for the current run.
Steps for preparing sample plates and loading them into the instrument.
Default well positions for sample injection on 48-capillary arrays.
Default well positions for sample injection on 96-capillary arrays.
Instructions for sealing and assembling sample plates prior to instrument loading.
Guidance on properly placing the assembled plates into the instrument's stacker.
Overview of the functionalities and benefits of the Thermo Fisher Connect feature.
Steps to create an account for the Thermo Fisher Connect service.
Procedure to link the instrument to the user's Thermo Fisher Connect account.
Configuring data storage paths and email notification settings for the Connect account.
How to monitor instrument runs remotely using the InstrumentConnect web interface.
Instructions for monitoring instrument runs using a mobile device application.
Accessing and viewing instrument notifications and alerts through the cloud platform.
Information on managing administrator roles and functions within Thermo Fisher Connect.
Procedure to create a results group with default naming and storage conventions.
Steps to create and configure a plate record for instrument runs.
Explanation of different run modes and default injection configurations.
Detailed steps for running the instrument using the automated mode.
Instructions for operating the instrument in manual run mode.
Procedure to modify the injection order of plate quadrants.
How to move plates within the input stack while a run is in progress.
Using the software toolbar to control active instrument runs.
Methods for monitoring the real-time status of an ongoing instrument run.
Steps to add reinjections to an existing plate record for a run.
How to check the current operational status and calibration information of the instrument.
Accessing and reviewing the instrument's event log for error diagnosis.
Procedures for viewing run data using Capillary Viewer and Array Viewer.
Verifying autoextraction status and performing manual re-extractions.
Explanation of what a plate record is and its purpose in instrument operation.
Procedures for creating, editing, deleting, and exporting plate records.
How to search for existing plate records based on various criteria.
Instructions for importing plate records from a text file.
Understanding the purpose and configuration of results groups for data organization.
Procedures for creating, editing, duplicating, and exporting results groups.
Step-by-step guide for creating a results group with custom settings.
Customizing file naming conventions for sample data within results groups.
Overview of managing instrument protocols, including creation and editing.
Steps to create a new instrument protocol for run configuration.
Procedure for creating and configuring run modules for instrument operation.
Explanation of analysis protocols, particularly for sequence analysis.
Managing analysis protocols, including creation, editing, and deletion.
Detailed steps for creating a new analysis protocol.
Procedure for calibrating the instrument's spatial positioning for accurate data.
Steps for performing spectral calibration for DNA sequencing and fragment analysis.
Schedule of recommended daily, weekly, and monthly maintenance tasks.
Overview of the available maintenance wizards and their purposes.
Guidance on when to use specific maintenance wizards based on instrument conditions.
General instructions for cleaning the exterior surfaces of the instrument.
Procedures for replenishing or changing the polymer used in the instrument.
Steps for cleaning the polymer delivery pump and associated components.
Procedure for flushing the water seal trap in the polymer delivery pump.
Instructions for installing a new or used capillary array.
Proper methods for storing capillary arrays to maintain their integrity.
Detailed instructions for cleaning the instrument's detection cell.
Steps for safely shutting down the instrument for short periods of inactivity.
Guidelines for managing disk space for instrument data and the database.
Procedure for restarting the instrument and associated computer system to resolve issues.
How to access and view the instrument's event log for error diagnosis.
Guidance on locating and reviewing run log files for troubleshooting.
Information on the preparation and storage of Hi-Di Formamide.
Troubleshooting guide for interpreting and resolving issues indicated by front panel status lights.
Common issues and solutions related to instrument run failures or errors.
Troubleshooting specific problems encountered during sample re-extraction.
Diagnosing and resolving issues related to the service console software.
Addressing common problems encountered during spatial calibration.
Troubleshooting guide for spectral calibration failures and data issues.
General tips and common problems encountered during instrument operation.
Technical specifications including physical dimensions, weight, and power consumption.
Conditions for installation site, altitude, and operating environment.
Details on power supply, voltage, and current requirements for the instrument.
Information on power supply and data communication connections for the instrument.
Approximate run times for various fragment analysis modules.
Approximate run times for different sequence analysis modules.
List of supported dye sets and their corresponding calibration standards.
Specific details on G5 and G5-RCT dye sets for fragment analysis.
Catalog numbers for plates, bases, retainers, and septa.
Catalog numbers for various instrument consumables.
Catalog numbers for calibration standards for sequencing and fragment analysis.
Catalog numbers for fragment analysis size standards.
Explanation of hazard symbols, their meanings, and locations on the instrument.
Symbols related to instrument controls, power sources, and data connections.
Symbols indicating compliance with safety and regulatory standards.
General safety guidelines and warnings for operating the instrument safely.
List of related documents and their publication numbers.
Information on how to contact customer and technical support services.
Details regarding the limited product warranty terms and conditions.
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