Applied Biosystems 3730xl User Manual

Run module parameters
Parameter Name
Range Comment
Oven_Temperature 18℃–70℃ Temperature setting for the main oven throughout the run.
PreRun_Voltage 0–15 kV Pre run voltage setting before sample injection.
PreRun Time 1–1,800 seconds Prerun voltage time.
Injection_Voltage 0–15 kV Injection voltage setting for sample injection.
Injection_Time 1–90 seconds Sample injection time.
First_ReadOut_time 100–16,000
milliseconds
The interval of time for a data point to be produced.
First_ReadOut_time should be equal to
Second_ReadOut_time.
Second_ReadOut_Time 100–16,000
milliseconds
The interval of time for a data point to be produced.
Second_ReadOut_time should be equal to
First_ReadOut_time.
Run_Voltage 0–15 kV Final run voltage.
Voltage_Number_Of_Steps
[1]
0–100 steps Number of voltage ramp steps to reach Run_Voltage.
Voltage_Step_Interval
[1]
0–180 seconds Dwell time at each voltage ramp step.
Voltage_Tolerance
[1]
0.1–6 kV Maximum allowed voltage variation.
Current_Stability
[1]
0–2,000 μA Maximum allowed electrophoresis current variation. Current
fluctuations above this value will be attributed to air bubbles in
system and the voltage automatically powered off.
Ramp_Delay
[1]
1–1,800 seconds Delay During Voltage Ramp.
Data_Delay 1–1,800 seconds Time from the start of separation to the start of sample data
collection.
Run_Time 300–14,000 seconds Run time during which data is collected after Ramp_Delay.
[1]
D
o not change this value unless you are instructed to do so by Support.
Overview of analysis protocols (sequence analysis)
An analysis protocol contains all the seings that are necessary for sequence analysis
with Sequencing Analysis Software or SeqScape
™
Software.
• Basecalling seings—The basecaller, DyeSet le, and analysis stop point to be
used.
• Mixed Bases—(Optional) Mixed base identication and the percent value of the
second highest to the highest peak.
• Clear Range—The clear range to be used based on base positions, sample quality
values, and/or number of ambiguities (Ns) present.
Chapter 7 Cr
eate and manage instrument protocols, run modules, and analysis protocols
Overview of analysis protocols (sequence analysis)
7
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
87

Applied Biosystems 3730xl Specifications

General

Brand	Applied Biosystems
Model	3730xl
Category	Measuring Instruments
Language	English