d.
Empty the anode buer jar into an aqueous waste container.
5.
Add 67 mL 1X run buer to the jar.
6.
Put the anode buer jar on the instrument with the overow hole facing you.
7.
Verify that the meniscus of the buer is just below the red ll line on the jar.
8.
Verify that the electrode is immersed in the buer.
Check the polymer level
Check the level of polymer, then replenish if needed. Replace polymer if it has been
on the instrument for more than 1 week. See “Replenish or change polymer type“ on
page 119.
IMPORTANT! Allow polymer to reach room temperature before installing on the
instrument.
1
Electrode
2
Meniscus
Chapter 2 Prepare the instrument
Check the polymer level
2
34
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide