Perform spectral calibration for sequencing and fragment analysis
A spectral calibration creates a matrix that is used during a run to reduce raw data
from the instrument to the 4- or 5-dy
e data stored in the sample les.
Note: The run times listed below do not include the time for the oven to preheat.
Application Standard Polymer
Capillary array
l
ength
Run module Run Time (min)
[1]
Sequencing Sequencing
standard
POP-7
™
36 cm Spect36_SeqStd_POP7 60
50 cm Spect50_SeqStd_POP7 115
POP-6
™
36 cm Spect36_SeqStd_POP6 65
50 cm Spect50_SeqStd_POP6 145
Fragment
analysis
Matrix
standard
POP-7
™
36 cm Spect36_MtxStd_POP7 50
50 cm Spect50_MtxStd_POP7 60
[1]
The software may take up to 30 minutes to calculate the matrices after the run is complete.
• For each dye set–capillary array type–capillary array length combination
• After the laser or CCD camera has been realigned/replaced by a service engineer
• After the capillary array is changed or replaced
• If you see a decrease in spectral separation (pull-up and/or pull-down peaks)
• If you alter any condition (dye set, array type, array length, or polymer type)
Spectral
c
alibration
definition and run
time
When to perform
the calibration
Chapter 8 Calibr
ate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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