Applied Biosystems 3730xl User Manual

Calibrate the instrument
■
Perform spatial calibration ............................................. 91
■
Perform spectral calibration for sequencing and fragment analysis .......... 97
Perform spatial calibration
The software uses images collected during spatial calibration to establish a
relationship between the signal emied by each capillary and the position where it is
detected by the CCD camera.
Estimated run time is <5 minutes.
For all dye sets, perform a spatial calibration after you perform any of the following
actions.
• Remove or replace the capillary array
• Remove the capillary array from the detection cell block (even to adjust it)
• Move the instrument
• Move the array detection cell
Note: Failure to perform a new spatial calibration can result in poor data quality.
1.
In the navigation pane of the Data Collection Software, select GA
Instruments4 ga37304 Instrument name4 Spatial Run Scheduler.
2.
In the Spatial Run Scheduler view
,
select one of the following options
• Protocol4SpatialNoFill if the
capillaries contain fresh
polymer.
• Protocol4SpatialFill.
Note: You do not need to ll the
capillaries each time you perform a
spatial calibration.
3.
Click Start.
4.
Evaluate the calibration. See “Evaluate the spatial calibration data“ on page 92.
8
Spatial calibration
description and
run time
When to perform a
spatial calibration
Perform spatial
calibration
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
91

Applied Biosystems 3730xl Specifications

General

Brand	Applied Biosystems
Model	3730xl
Category	Measuring Instruments
Language	English