Applied Biosystems 3730xl User Manual

Table 7 Sequencing dye sets and standards
Kit used for sequencing Dye Set Calibration Standards
BigDye
™
Terminator v3.1
Cycle Sequencing Kit
Z_BigDyeV3 3730/3730xl DNA Analyzer
Sequencing Standards,
BigDye
™
Terminator v3.1
BigDye
™
Direct Cycle
Sequencing Kit
Z_BigDyeV3
BigDye
™
Terminator v1.1
Cycle Sequencing Kit
E_BigDyeV1 3730/3730xl DNA Analyzer
Sequencing Standards,
BigDye
™
Terminator v1.1
Table 8 Fragment analysis dye sets and matrix standards
Dye Set Calibration Standards
G5 DS-33
G5-RCT (reduced cross-talk)
[1]
J6 DS-36
Any4Dye, Any5Dye Custom
Any4Dye-HDR (high dynamic range)
[2, 3]
Custom
Any5Dye DS-02 for SNaPshot
™
applications
Custom
[1]
U
se for fragment analysis applications on 96-capillary arrays.
[2]
Signal intensity is reduced by approximately half relative to the standard dye sets, along with a minimal
reduction in the noise, resulting in a very slight decrease in the signal/noise ratio when compared to data
generated using the standard dye sets
[3]
When using this dye set, perform spectral calibrations each time the capillary array is replaced or moved
within the detection cell
• 384- or 96-Well Reaction Plate w/Barcode
• Multichannel pipeor
• Plate retainer
– Plate septum with black plate base
or
– Heat-seal with gray plate base
• Hi-Di
™
Formamide
• Heated block or thermal cycler
• Container with ice
• Centrifuge with microplate adapter
• Microcentrifuge
• Vortex
• Gloves
Supported dye
sets and
calibration
standards
Required
mat
erials
Chapter 8 Calibrate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
98
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide

Applied Biosystems 3730xl Specifications

General

Brand	Applied Biosystems
Model	3730xl
Category	Measuring Instruments
Language	English