Seal and assemble plates
WARNING! Do not use warped or damaged plates.
Seal the plate with a heat-seal or septum.
Option Description
Heat seal Follow your thermal sealer instrument instructions.
Septum
seal
1. Place the plate on a clean, level surface.
2.
Place a new septum flat on the plate.
3. Align the holes in the septum strip with the wells of the plate, then
firmly press downward onto the plate. Ensure that the septum is
seated and aligned (see Figure 8).
• The septa lie flat against the plate. You should not feel any lumps
or raised edges.
• The septa are inserted straight into the wells. You should not see
any bent or crooked duckbills when viewing the plate from above.
Figure 8 Exampl
es of bad and good septa alignment
1
Septum and well are not aligned
2
Septum and well are not aligned
3
Septum and well are not aligned
4
Septum and well are properly aligned
Seal and prepare
the plate
assemblies
Chapter 2 Prepare the instrument
Seal and assemble plates
2
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
41
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