Contents
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CHAPTER 1 Instrument and software description ................... 10
Overview ...................................................................... 11
Parts of the instrument ......................................................... 12
Instrument interior components ................................................. 13
Instrument front panel indicators ................................................ 14
Consumables and usage limits .................................................. 16
Important notice regarding use of consumables that exceed supported limits ......... 16
Computer and software requirements ............................................ 17
Theory of operation ............................................................ 17
Preparing samples ......................................................... 17
Preparing the instrument ................................................... 18
During a run .............................................................. 18
Results ................................................................... 18
Overview of the software ........................................................ 19
Use the instrument with Thermo Fisher Connect ................................... 22
Secondary analysis software .................................................... 23
Data Collection software terminology ............................................. 24
Workflow for a typical run ....................................................... 26
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CHAPTER 2 Prepare the instrument .................................. 27
Power on the computer and the instrument ....................................... 27
Start the software .............................................................. 28
Prepare buffer and fill the reservoirs ............................................. 29
Required materials ........................................................ 29
1X run buffer storage ...................................................... 29
When to change the 1X run buffer ............................................ 30
Prepare the 1X run buffer ................................................... 30
Fill the water and buffer reservoirs .......................................... 30
Place reservoirs into the instrument .............................................. 32
Fill the anode buffer jar ......................................................... 33
Check the polymer level ........................................................ 34
Check for bubbles in the pump system ............................................ 35
Check the active spectral calibration ............................................. 36
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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