1.
Examine the peaks of the spatial calibration.
A prole is acceptable if the peaks meet the following conditions.
• Peaks in the prole are approximately the same height.
• The peak height increases at the beginning and the end of the spatial prole.
Note: If you are using a 96-capillary array, a small peak may appear in the left
side of the prole. The peak is normal.
Figure 13 Passing spectral profiles
For additional information, see “Examples of passing and failing spatial
proles“ on page 94.
A prole is not acceptable if the peak heights are irregular. See “Spatial
calibration troubleshooting“ on page 138.
2.
Verify that an orange cross appears at the top of each peak in the prole.
Expand the prole if needed. See “Magnify the spatial prole“ on page 96.
Figure 14 P
eak that does not have orange cross
1
No orange cross
Evaluate the
spatial calibration
data
Chapter 8 Calibrate the instrument
Perform spatial calibration
8
92
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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