Applied Biosystems 3730xl User Manual

Attribute Acceptance criteria Example
Peak morphology in
the spectral profile
(intensity vs pixel)
• No gross
overlaps, dips, or
other
irregularities
• Peaks separate
and distinct
• Peak apexes are
separate and
distinct (the tails
will overlap)
1
1
Dip
Note: The peak
morphol
ogy of G5
(shown to the right,
top), F, and J6 (shown
to the right, bottom)
may not be as
rounded and
symmetrical as the
peak morphology for
other dye sets (shown
above) due to the
effect of variable
binning (a feature that
reduces signal
variation between
dyes of different
fluorescent
efficiencies).
1.
Click-drag the cursor to create a box around the area of interest.
Magnify the
spectr
al profile
Chapter 8 Calibr
ate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
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3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide

Applied Biosystems 3730xl Specifications

General

Brand	Applied Biosystems
Model	3730xl
Category	Measuring Instruments
Language	English