2.
In the Events Messages section of the window, view the status of each capillary.
1
Pass/fail status
2
q value (Spectral Quality Value)
3
c value (Condition Number)
Spectral Quality Value
A spectral Quality V
alue reects the condence that the individual dye emission
signals can be separated from the overall measured uorescence signal. It is a
measure of the consistency between the nal matrix and the data from which it was
computed. A Quality Value of 1.0 indicates high consistency, providing an ideal
matrix with no detected pull-up/pull-down peaks.
In rare cases, a high Quality Value can be computed for a poor matrix. This can
happen if the matrix standard contains artifacts, leading to the creation of one or more
extra peaks. The extra peaks cause the true dye peak to be missed by the algorithm,
and can lead to a higher Quality Value than would be computed with the correct
peak. Therefore, it is important to visually inspect the spectral calibration prole for
each capillary.
Condition Number
A Condition Number indicates the amount of overlap between the dye peaks in the
uorescence emission spectra of the dyes in the dye set.
If there is no overlap in a dye set, the Condition Number is 1.0 (ideal conditions), the
lowest possible value. The condition number increases with increasing peak overlap.
Quality (Q) value and condition number passing ranges
Dye Set
Quality Value minimum
Default Condition Number
Range
Z_BigDyeV3 >0.93 2.5–4.5
E_BigDyeV1
>0.95
3.0–5
G5
8.5–13.5
G5-RCT
J6
>0.8
5.0–8.0
Any4Dye 1.0–20.0
Chapter 8 Calibrate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
104
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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