Applied Biosystems 3730xl User Manual

Polymer
Capillary
Array
Length (cm)
Sequencing Run Sequencing Run Modules
Approximate
Run Times
[1]
(minutes)
KB
™
Basecaller
QV20 CRL
(Bases)
[2]
POP-6
™
50 Long read LongSeq50_POP6 150 ≥600
36 Standard read StdSeq36_POP6 60 ≥500
[1]
T
hese approximate run times assume oven temperature has reached run temperature
[2]
Contiguous read length with 98.5% basecalling accuracy, and less than 2% N’s, using pGEM
™
-32f (+) as template.
[3]
Run the BDx utility after installation or after preventative maintenance is performed.
[4]
Time stated for 400 bases. Module can be customized to run 200-400 bases.
IMPORTANT! Use BDX run modules only if y
ou prepare samples with BigDye
XTerminator
™
Purication Kit. Use non-BDX run modules for samples puried with
other methods.
Appendix C Run modules
Sequence analysis run times
C
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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Applied Biosystems 3730xl Specifications

General

Brand	Applied Biosystems
Model	3730xl
Category	Measuring Instruments
Language	English