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CHAPTER 8 Calibr
ate the instrument ................................. 91
Perform spatial calibration ...................................................... 91
Spatial calibration description and run time ................................... 91
When to perform a spatial calibration ........................................ 91
Perform spatial calibration ................................................. 91
Evaluate the spatial calibration data ......................................... 92
Examples of passing and failing spatial profiles ................................ 94
Magnify the spatial profile .................................................. 96
Perform spectral calibration for sequencing and fragment analysis .................. 97
Spectral calibration definition and run time ................................... 97
When to perform the calibration ............................................. 97
Supported dye sets and calibration standards ................................. 98
Required materials ........................................................ 98
Prepare the spectral calibration standard ..................................... 99
Create a spectral instrument protocol ........................................ 99
Create a spectral calibration plate .......................................... 100
Run the spectral calibration plate ........................................... 101
Place plate assemblies into the instrument .................................. 102
View the Pass/Fail status after the run ...................................... 103
Evaluate the spectral calibration data ....................................... 105
Magnify the spectral profile ................................................ 108
Examples of passing sequencing spectral calibrations ........................ 110
Example of a passing fragment analysis spectral calibration ................... 111
Select a previous calibration as the active spectral calibration .................. 111
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CHAPTER 9 Maintenance ............................................. 113
Maintenance schedules ........................................................ 113
Daily maintenance ........................................................ 113
Weekly maintenance ...................................................... 114
Monthly maintenance ..................................................... 115
As-Needed maintenance .................................................. 115
Wizards types and functions .................................................... 116
Typical conditions for using maintenance wizards ................................. 117
Effects of the instrument door state on wizard tasks .......................... 118
Manual control of autosampler content ...................................... 118
Clean the instrument .......................................................... 119
Replenish or change polymer type .............................................. 119
Before using the polymer .................................................. 119
Replenish the polymer .................................................... 119
Change polymer type ...................................................... 120
Contents
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3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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