LB 9507 Chapter 7: Quantitative Measurements (LIA...)
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L69
CALCULATING SPLINE FUNCTION ...
SMOOTHING FACTOR 1
The cubic spline polynomials are calculated as a connecting curve
between the individual standard measured values.
The logarithmic values of the standard concentrations are plotted
along the X-axis in relative units, the first standard being located
at the origin and the highest at the end of the plotting range.
Depending on what is defined in the protocol (logit/log or log-log),
the Y-axis shows either the logarithm or the logit function of the
RLU values standardized to the highest standard.
If the standard curve fails to meet certain quality criteria (no
balance point, no or one point of inflection in the lin/log
representation of the values), Lumat will automatically smooth the
curve and display the smoothing factor used.
L70
STANDARD CURVE:
CONTINUE CHANGE PRINT
At this point the standard curve is calculated and its characteristic
data are printed out.
<PRINT> creates a graphic plot of the curve and the following
appears on the display:
L70a
PLOTTING CURVE ...
The program then returns to display L70.
L70
STANDARD CURVE:
CONTINUE CHANGE PRINT
<CHANGE> allows you to correct the current standard curve. The
program moves to display 71.
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