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Molecular Devices FLIPR Tetra User Manual

Molecular Devices FLIPR Tetra
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Contents
10 0112-0109 H
Appendix B—Bar Code Specifications . . . . . . . . . . . . . . 271
About Bar Codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Bar Code Recommendations . . . . . . . . . . . . . . . . . . . 271
Bar Code Specifications . . . . . . . . . . . . . . . . . . . . . . . 272
Appendix B: Data Processing Algorithms . . . . . . . . . . . 273
Hypothetical Experiment . . . . . . . . . . . . . . . . . . . . . . . 273
Crosstalk Correction . . . . . . . . . . . . . . . . . . . . . . . . . . 274
Determining Crosstalk Correction . . . . . . . . . . . . . . . . 274
Spatial Uniformity Correction . . . . . . . . . . . . . . . . . . . . 275
Determining Spatial Uniformity Correction . . . . . . . . . . 275
Negative Control Correction . . . . . . . . . . . . . . . . . . . . . 276
Determining Negative Control Correction. . . . . . . . . . . 277
Positive Control Scaling . . . . . . . . . . . . . . . . . . . . . . . . 278
Determining the Positive Control Scaling . . . . . . . . . . . 278
Subtract Bias . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279
Determining Subtract Bias . . . . . . . . . . . . . . . . . . . . . 279
Response Over Baseline . . . . . . . . . . . . . . . . . . . . . . . 280
Determining Response Over Baseline Correction. . . . . . 280
Appendix C: Consumables and Accessories . . . . . . . . . 283
FLIPR Tetra System Accessories . . . . . . . . . . . . . . . . . . 283
Field Installations . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
Pipettor Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
Optics Consumables . . . . . . . . . . . . . . . . . . . . . . . . . 284
Pipetting Consumables . . . . . . . . . . . . . . . . . . . . . . . 285
Cell Reservoir Consumables . . . . . . . . . . . . . . . . . . . . 286
Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
96-Well Read Plates . . . . . . . . . . . . . . . . . . . . . . . . . 287
96-Well Read Plate Masks . . . . . . . . . . . . . . . . . . . . . 287
96-Well Source Plates . . . . . . . . . . . . . . . . . . . . . . . . 288
384-Well Read Plates . . . . . . . . . . . . . . . . . . . . . . . . 289
384-Well Source Plates . . . . . . . . . . . . . . . . . . . . . . . 290
1536-Well Read Plates . . . . . . . . . . . . . . . . . . . . . . . 290
1536-Well Source Plates . . . . . . . . . . . . . . . . . . . . . . 291
Source Reservoirs. . . . . . . . . . . . . . . . . . . . . . . . . . . 291
Assays Performed on the FLIPR Tetra System . . . . . . . . 291
Calcium Flux Consumables. . . . . . . . . . . . . . . . . . . . . 291
FLIPR Membrane Potential Assay Kit Consumables . . . . 293

Table of Contents

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Molecular Devices FLIPR Tetra Specifications

General IconGeneral
BrandMolecular Devices
ModelFLIPR Tetra
CategoryLaboratory Equipment
LanguageEnglish

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