FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 29
Figure 2-7 Cell Suspension Module connections.
Pin Tool Head
Pin tools are blocks of solid or slotted pins, where the pins replace the
hollow tips used with a standard pipettor. The pins use capillary action
to pick up and transfer liquid from one plate to another. Their ability to
accurately and reliably transfer compounds in nanoliter volumes allows
users to supply test compounds in 100% DMSO solution, removing the
need to prepare intermediate dilution plates.
The volume that each pin picks up is determined by the size of the pin
(and, if slotted, the size of the slot) and the withdrawal speed of the pin
from the liquid—a faster removal speed leaves more liquid on the pin.
Pins for the 384 pin tool are supplied in four sizes, giving a total range
(across all these sizes) from 84 nL to 656 nL. The 1536 pin tool has
seven different pin sizes, giving a total range from 19 nL to 117 nL.
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