FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 119
Mix Fluid (Pin Tool)
Fluid mixing with a pin tool consists of a sequence of up and down
motions of the pin block.
Item Description
Mix Plate Select the plate that has the fluid to be mixed. Typically,
it is a source plate from which you will be transferring
fluid.
Top Height Enter the height (in μL) for the top of the stroke during
mixing.
Quadrant If appropriate, select a quadrant in which fluid will be
mixed.
Note: Quadrant selection is only available when
liquid is dispensed to a plate using a pipettor head
of lower density, for example, 1536-well plate
using a 384 pipettor head.
Bottom Height Enter the height (in μL) for the bottom of the stroke
during mixing.
Strokes Enter the number of times the pins move within the mix
step. One stroke is equal to one up-stroke and one
down-stroke.
Down Speed Enter the speed (in mm/s) of the down-stroke
Removal
Speed
Enter rate (in mm/s) at which pins are pulled from the
well immediately after the mix. This value is adjusted
for fluid viscosity or fluid volume in the well. The speed
of removal determines the volume of fluid remaining on
the pins.
Up Speed Enter the speed (in mm/s) of the up-stroke. This is not
the rate which pins will be removed from the well.
Pause in Well Enter a duration to hold the pin tool in the well plate
once mixing is completed, before withdrawal.
Unload Pin Tool Check to have the pin tool unloaded after mixing.
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