Molecular Devices FLIPR Tetra User Manual

Running an Experiment
188 0112-0109 H
Luminescence Assay Protocol
This section provides the following information and procedures:
• Materials required for the assay
• Cell preparation guidelines
• Coelentrazine loading procedure
• Running the assay on FLIPR
®
Tetra System
• Optimizing tips for Luminescence assays
Required Materials
The following materials are required for running a luminescence assay:
Table 8-3 Cell Preparation for Aequorin Assays.
Addition Parameters 96-Well Plate 384-Well Plate
Compound Addition Volume (μL) 50 25
Dispense Speed (μLs)
Adherent Cells
50–100 10–50
Dispense Speed (μL/s)
Non-adherent Cells
10–50 5–20
Note: The references to the instrument assume the Aequorin ICCD
camera option is present along with the Cell Suspension option.
Item Source
FLIPR
®
Tetra System with Aequorin,
ICCD camera option and Cell
Suspension option
Molecular Devices
Clear, flat-bottom, black wall 96-,
384-, 1536-well plates
See Appendix C
Clear polypropylene source plates (for
adherent protocols)
See Appendix C
Black FLIPR
®
System pipette tips, 96-
or 384-well or 1536 tip block and
gaskets
See Appendix C
Test compounds Specific to receptor
Cells in suspension Optional
Coelenterazine Specific to Photoproteins
Growth medium Major Laboratory Supplier (MLS)

Molecular Devices FLIPR Tetra Specifications

General

Brand	Molecular Devices
Model	FLIPR Tetra
Category	Laboratory Equipment
Language	English