FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 71
Processes can be added to the end of a protocol, or, using the right
edge of a new process you drag over, inserted between other processes
already in the protocol, providing you do not attempt to insert between
processes that are internally linked, for example, a Transfer Fluid and
associated Mix with TF.
As each new process is dragged to the Experiment window, a new page
with configuration options for the process is added to the file. The new
page is automatically displayed.
Similarly, as you click on the processes above, the sheet associated
with that process, containing its configuration settings, comes forward.
Deleting Processes from the Protocol
To delete processes that are not connected click on the process icon in
the Experiment window and click the Delete key.
To delete a connected process—Mix with TF or Read with TF—go to
the Transfer Fluid process that it is associated with and uncheck the
option for the process.
Process Icon Colors
The Settings and Analysis processes’ icons are green to indicate that
they are required to run an experiment. These processes’ are always
present and cannot be deleted.
The Transfer Fluid, Mix Fluid, Wash Tips or Pins, Wash Reservoir,
Blot Pins, Pause Pipettor and Finish with Source processes icons
are blue to indicate that they are liquid handling steps. These steps are
always in series with each other; no two of them will ever occur
simultaneously.
The Read process icon is orange to indicate that it will occur in parallel
with any liquid handling process, if possible. However, a read can be
added through the Transfer Fluid process to indicate the liquid
handling will occur at a defined time in the experiment. These linked
processes will not begin until all read and liquid handling steps prior to
the Transfer Fluid linked with Read with TF are complete.
When Mix with TF or Read with TF processes are created (from the
options in a Transfer Fluid step), the icons of all the connected
processes change to purple, to show the linkage.
Settings Process
Settings is the first process in all protocols. Use it to define read modes,
plate positions, and data directories. You can also configure automatic
printing of selected results, automatic file naming, and set plate
temperature.
Select the green Settings icon in the Experiment window to view the
Settings page where the settings are made.
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