ScreenWorks Software Overview
72 0112-0109 H
Setup Read Mode
The Setup Read Mode table at the top of the Settings page displays
settings for up to four read modes that the FLIPR
®
Tetra System can
perform in a single experiment.
Each read mode defines:
• Reading mode (fluorescence or luminescence)
• Excitation and emission wavelengths (i.e., a pair of LED banks
and emission filter)
• Camera gain
• Camera exposure time
• Excitation intensity
• Gate open (ICCD camera only)
Molecular Devices FLIPR
®
Calcium and Membrane Potential Assay Kits
use a single read mode, but ratiometric assays, such as the Voltage
Sensor Probes, require two read modes. In these cases the instrument
alternates between the two read modes for each time point, outputting
two distinct sets of readings, one for each mode. ScreenWorks
Software can then automatically calculate the ratio of the readings from
each mode, for each time point.
If you open a protocol file that uses read modes not available under the
present configuration of your instrument, the unavailable read mode
rows are displayed in red.