Running an Experiment
164 0112-0109 H
Setting Up an Assay Protocol
An effective way to reduce time and ensure consistent screening
parameters is to preconfigure protocol (*.fmp) files. Some users define
and save protocol files for each cell line, project or user.
Saved protocol files are opened from File > Open > Protocol File. In
addition, the last 10 files (6 data and 4 protocol) opened in
ScreenWorks Software are listed in the bottom portion of the File
menu.
Creating a Protocol File
A protocol file defines experiment parameters:
• Instrument conditions, for example, read mode, plate positions,
file names and file storage.
• Assay steps, for example, fluid transfer and reads.
• Analysis options, for example, groups, corrections and export
parameters.
Protocols are created by an easy drag-and-drop operation that moves
experimental processes from the Process Explorer to the Experiment
window.
Defining the Settings Process
In the Settings process of the Experiment window, select the
appropriate parameters for Read Mode, Plate Position, Data File
Name and Folders. For a detailed explanation of the Settings process
and its options, Settings Process on page 71.
Creating the Assay Sequence
Select the desired processes from the Process Explorer and drag
them to the desired sequence position in the Experiment window.
Click on the process icon you want to define (for example, Transfer
Fluid), and then enter the desired parameters. For more information
on the available protocol processes and their options, see Chapter 5:
ScreenWorks Software Overview on page 47.
Note: The protocol processes are executed in a left-to-right order
until there is a combined process of Transfer Fluid and Read with
TF. After baseline imaging, all pipetting actions and imaging occurs in
parallel.
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