FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 37
Figure 2-9 LED configuration.
Emission Filters
A three-position filter slider holding up to three 60 mm diameter
interference filters is located in front of the CCD camera. The slider can
be alternated in front of the camera to separate out the emission band
of the dye being used. These filters can be used with a single excitation
wavelength or can be paired up with additional emission filters and
LEDs in a ratiometric experiment, for example, as excitation LEDs
alternate between two different wavelengths, filters change at the
same time so that each image taken by the camera matches the right
emission filter with the excitation LED bank. The most common FLIPR
®
Tetra System
configuration is a LED excitation wavelength of 470–495
nm with a 515–575 nm bandpass emission filter. For luminescence
experiments it is also possible to run without the filter.
Emission filters are user-changeable in approximately 5 minutes; see
Changing Filters on page 141 for instructions. Once installed, filters are
mechanically sensed and the filter configuration can be viewed in the
Instrument Configuration panel in ScreenWorks Software. The
instrument will prompt you to calibrate a new emission filter with
respect to an LED module if they are intended to be used in an
experiment.
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