FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 215
Troubleshooting the Yellow Plate
Table 9-6 Troubleshooting the Yellow Plate
Symptom or Error
Message
Possible Causes Solutions
“Saturation detected,
data may be invalid.”
Incorrect settings. Set excitation intensity to
80%, exposure length to
0.1 s and for EMCCD gain to
80, for ICCD gate to 3%. If
condition repeats, lower
excitation intensity.
Incorrect LED and/or
emission filter choice.
Check to make sure the
appropriate LED and
emission filters are
selected.
Emission optics are
dirty.
Make sure no dust is on
emission filters.
Bottom of Read plate is
dirty.
Make sure no dust or
fluorescent compounds are
on the bottom of the Read
plate.
Wells are cut off in
Image Viewer.
Plate not properly
placed in Read
position.
Make sure plate is properly
placed and indexed in the
Read position.
Dirty/misaligned
optics.
Contact Technical Support
at
+1-800-635-5577.
Fluorescence counts are
0.
LED power is off. Increase excitation intensity
of LEDs.
Make sure
Excitation/Emission
Wavelength in the read
mode does not have NONE
for the excitation
wavelength.
Camera failure. Contact Technical Support
at
+1-800-635-5577.
Signal test was not
initiated.
Select Test Signal in the
Signal Test dialog box.
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