FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 117
Mix Fluid Process
Fluid mixing steps can be added to protocols as independent protocol
steps—Mix Fluid—or linked to a Transfer Fluid step—Mix with TF.
Independent Mix Fluid steps can be configured to mix fluid in source or
read plates, while Mix with TF steps immediately follow the Transfer
Fluid step they are linked to, mixing fluid in the read plate where
compound was dispensed.
Different mix options are offered for standard pipettors and the pin tool
(described in different sections below).
Mix Fluid (Standard Pipettor)
Fluid mixing with a standard pipettor consists of a series of aspirations
and dispenses in and out of the tips.
Read Check this box to link a Read process to the Transfer
Fluid process.
This automatically adds a Read with TF step to the
protocol, and opens the configuration page for that
step.
See Read Process on page 120 for more information
about read process options.
Note: This option is required if you intend to
read a plate while transferring liquid during a
kinetic cell-based assay (for example, calcium
mobilization).
Mix Fluid Check this box to link a Mix Fluid process to the
Transfer Fluid process.
This automatically adds a Mix with TF step to the
protocol, and opens the configuration page for that
step.
See Mix Fluid Process on page 117 for more information
about mixing options.
Unload Pin Tool Automatically unload the pin tool after the dispense.
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