Running an Experiment
170 0112-0109 H
3. Click the Run button on the toolbar to start the program.
The upper and lower doors automatically lock before any part in
the FLIPR
®
Tetra System tower moves. These doors must
remain closed until the end of the program including the
duration of the pipettor tip wash.
FLIPR Calcium Assay Kit Protocol
This section provides the following information and procedures:
• Materials required to run the calcium mobilization using the
FLIPR
®
Calcium Assay Kit
• Cell preparation guidelines
• Dye loading procedure
• Troubleshooting tips for intracellular calcium assays
Required Materials
The following items are required for running the FLIPR
®
Calcium Assay
Kit:
Item Source
FLIPR
®
Tetra System with Calcium Optics Kit
installed (default with instrument):
FLIPR
®
Tetra System LED Module 470–495 nm
FLIPR
®
Tetra System Emission Filter 515–575
nm
Molecular Devices
See Appendix C.
FLIPR
®
Calcium Kits (one of the following):
FLIPR
®
Calcium Assay Kit, explorer
FLIPR
®
Calcium Assay Kit, bulk
FLIPR
®
Calcium 3 Assay Kit, express
FLIPR
®
Calcium 3 Assay Kit, explorer
FLIPR
®
Calcium 3 Assay Kit, bulk
FLIPR
®
Calcium 4 Assay Kit, express
FLIPR
®
Calcium 4 Assay Kit, explorer
FLIPR
®
Calcium 4 Assay Kit, bulk
FLIPR
®
Calcium 5 Assay Kit, express
FLIPR
®
Calcium 5 Assay Kit, bulk
FLIPR
®
Calcium 5 Assay Kit, explorer
FLIPR
®
Calcium Evaluation Kit
Molecular Devices
P/N R8041
P/N R8033
P/N R8108
P/N R8091
P/N R8090
P/N R8143
P/N R8142
P/N R8141
P/N R8187
P/N R8186
P/N R8185
P/N R8172
Clear, flat-bottom, black-wall or clear-wall
1536-, 384- or 96-well plates
See Appendix C.
Clear, polypropylene source plate See Appendix C.
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