FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 73
Edit Read Mode
To enable a read mode, click the check box in the far left of the Set
Read Mode table. Then double-click in the row or select the row and
press the Edit Mode button below the table to configure it. The Edit
Read Mode dialog has the following fields:
Item Description
Read Mode
Name
Enter a name for the selected read mode, if desired.
Reading Mode Select Fluorescence or Luminescence.
Excitation/
Emission
Wavelength
Select the desired excitation LED and emission filter
wavelength pair from the drop-down list.
Note: Only calibrated wavelength pairs are available
when the software is in Online mode; see Recalibrating
the Optics on page 150.
NONE instructs the instrument to not turn on any LEDs or
use an empty emission filter position. These are typically used
when performing luminescence experiments.
Excitation
Intensity
Select a value from the list to regulate the intensity of light
emitted by the LED bank for a given Fluorescence read. The
range is 20–100%.
Exposure
Time
Enter a time (in seconds) to regulate the time that light is
collected and measured by the camera. A longer exposure
increases signal intensity.
Exposure time affects the read interval for data collection. For
instance, an exposure time of 0.4 s, added to a required 0.1 s
camera integration time, makes the highest update frequency
0.5 s.
The range is 0.05 s to 30 s.
Camera Gain Select a value from the list to regulate the amplification of the
camera power. Increasing the camera gain increases the
signal. The range for the Standard EMCCD camera is 1–240.
With the Aequorin ICCD camera installed, the Fluorescence
read mode is preset to 2000, whereas in Luminescence mode
the range is 2000–280,000.
Camera Gate This option is only available with the Aequorin ICCD camera in
Fluorescence mode. It further regulates the signal intensity by
selecting the percentage of each frame captured.
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