FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 19
them. Plates can be loaded manually through the upper compartment
door, prior to an experiment, or robotically, as the experiment proceeds
, using the TETRAcycler.
The five positions of the stage are, from left to right:
• Position 1: Tips and/or Source Plate 1
• Position 2: Source Plate 2
• Position 3: Read Plate
• Position 4: Cell Reservoir and/or Source Plate 3
• Position 5: Tip Washer
Figure 2-2 The five-position stage.
Positions 1, 2 and 4 take standard, low volume, deep well and reservoir
source addition plates.
Tip loading and unloading occurs in Position 1, but this position can
double as a source plate position once tips are removed.
Position 3 opens to the optics chamber below for excitation of
fluorophores in read plate wells and emission reading.
Position 4 can be used for the Cell Reservoir included with the Cell
Suspension option. A single Cell Reservoir is compatible with all of the
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