FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 195
Loading Coelenterazine
Conditions to check:
• Loading buffer (inclusion of FBS or BSA, type of buffer).
• Dye loading temperature and duration below 22 °C.
• Coelenterazine specific for Photoproteins.
• Presence of additives (for example, probenecid and pluronic acid
in intracellular calcium assays).
Source Plate
Conditions to check:
• Type of buffer used should match coelenterazine buffer.
• For suspension assay, prepare 2x compound directly in read
plate.
• For adherent assays, prepare a polypropylene plate with 2x
compound.
• Volume of fluid in source plate in relation to required transfer
volume and plate dead volume.
To Next Page
Loading...
Need help?
General
