FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 259
the parameters, their purpose, and the appropriate range for the
commands.
Table A-1 Washtips<TAB>Fluid Type, Wash Cycles, Volume/Stroke,
Aspirate Speed, Pump Speed, Strokes, Hold Time, Dispense
Speed<CR> command parameters
Parameter Description
Acceptable values or range
96-well
head
384-well
head
1536-
well head
Fluid type Indicates which wash
fluid bottle to be used.
a, b
Wash Cycles Number of wash
reservoir fill/drain
cycles to complete.
The requested # of
strokes will be
repeated for each
cycle.
1,2,3,4,5
Volume/stroke Volume to be drawn
into the pipette tip in
each stroke.
5–206
(double)
1–28
(double)
.1–3
(double)
Aspirate speed Pipettor aspiration
speed in micro liters
per second.
5.00,
10.00,
20.00,
30.00,
40.00,
50.00,
75.00,
100.00,
125.00
0.50, 1.00,
2.00, 5.00,
7.00,
10.00,
15.00,
20.00,
25.00,
30.00
0.25, 0.50,
0.75, 1.00,
1.25, 1.50,
2.00, 3.00,
5.00, 10.00
Pump speed Speed to run the
pumps which fill and
drain the wash
reservoir. Two speeds,
fast and slow, are
available. Slow is
recommended for use
with wash solvents
which tend to bubble.
fast, slow
Strokes Number of times
which the pipettor will
fill and empty with the
requested volume in
each wash cycle.
1–20
(integer)
Hold time Pause time in seconds
at the top of the
stroke of the pipettor.
0–15
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