Thermo Scientific QuantStudio 1 - Page 10

Instrument filters and supported dyes
System dyes
The instrument use a coupled three-color lter set that supports the dyes shown in the
following table and gure. For more information about available spectral dye
calibration kits, contact Support.
Peak
filter
Color
Filter wavelength
(nm)
[1]
Factory-calibrated
dyes
Example
custom dyes
Excitation Emission
x1-m1 Blue 470 ± 15 520 ± 15
FAM
™
,
SYBR
™
Green
SYT09
x2-m2 Green 520 ± 10 558 ± 12 VIC
™
JOE
™
,
HEX
™
, TET
™
[2]
x4-m4 Orange 580 ± 10 623 ± 14 ROX
™
, JUN
™
Texas Red
™
[1]
The central wavelengths are the optimized wavelengths.
[2]
The HEX
™
and TET
™
dyes from Thermo Fisher Scientific fall within the emission wavelength range of the
system, therefore they can be added and adapted for use on the instrument.
Filters
Wavelength
(nm)
1
2
3
x1-m1 x2-m2 x4-m4
Emission
Spectra
1
x1-m1 — FAM
™
, SYBR
™
Green
2
x2-m2 — VIC
™
3
x4-m4 — JUN
™
, ROX
™
, Texas Red
™
Custom dyes
The instrument can run assays designed with custom dyes. Custom dyes include the
following:
• Dyes that are not supplied by Thermo Fisher Scientic.
• Dyes or formulations of dyes that are not system dyes for the instrument.
To use a custom dye on the instrument, review the following requirements.
• Calibrate the instrument for the custom dye (see “Calibrate custom dyes“ on
page 62).
• Ensure that the custom dye excites and emits within the wavelength range of the
lters (see the lter-wavelength table in “System dyes“).
• Select a custom dye that does not overlap with other dyes used in the run (see the
lter-wavelength table in “System dyes“).
Chapter 1
Product information
Instrument hardware description
1
10
QuantStudio
™
1 Real-Time PCR System Installation, Use, and Maintenance Guide