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Agilent Technologies 1290 Infinity II 2D-LC - Page 149

Agilent Technologies 1290 Infinity II 2D-LC
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2D-LC User Guide 149
6 Method Parameters
Set the 2D-LC Method parameters
4 Select Threshold, Slope or Threshold and Slope from the Peak detection mode
drop-down list.
[OPTIONAL]
a Set Threshold.
In
Threshold
mode, the 2D-LC Valve is triggered on the threshold of the
detector signal. The threshold value is given as mAU value. When the UV
signal rises above this value, with a certain delay the 2D-LC Valve is
triggered and switches to cut the fraction. The 2D-LC Valve will switch to the
next position when the UV signal falls below this value or the cut size elapse.
[OPTIONAL]
b Or set the Slope.
In Slope mode, the 2D-LC Valve is triggered on the slope of the detector
signal. Adequate values for Up Slope and Down Slope can be specified in
the corresponding fields. This value is given as mAU/second. The 2D-LC
Valve switches when the up slope exceeds the given value. Cutting ends
when the slope passes a minimum and then rises above the down slope
value or the cut size is elapsed.

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