Agilent Technologies 4150 TapeStation system User Manual

Agilent 4150 TapeStation System Manual 207
Troubleshooting
10
Troubleshooting RNA Applications
A split or additional
unexpected peak is
evident in the RNA
ladder
Insufficient
denaturation of the
RNA ladder
Some peaks may have
partially reverted back
to its original,
non-denatured
conformation which
results in characteristic
double peaks
Ensure the correct denaturation conditions
when preparing the ladder. Ladder and samples
should be kept on ice, and run within 2 hours of
denaturation (see “Heat Denaturation” on
page 99)
Wrong peak assigned In rare cases, shoulders
of peaks can get
assigned as ladder
peaks
Delete the wrong peaks and assign correct
ladder peaks if needed.
See the Agilent Information Center for more
information
Additional bands are
present at the top of
the gel image
Incorrect ScreenTape
device storage
This peak is
comparable to the
'Well' peak in the
Genomic DNA assay
(“Genomic DNA
Assay” on page 178)
and can occur if the
ScreenTape device
stored incorrectly
Follow the specified storage conditions for the
assay (see Ta bl e 3 on page 64). Do not leave
ScreenTape devices for prolonged period of time
at room temperature. See the Agilent
Information Center for more information
The sample may be too
concentrated for the
application
A too high
concentrated sample
can result in artifacts in
the gel
Either dilute or concentrate your sample until it
is within the recommended range for
application as stated in the assay Quick Guide
(see “Quantitative range” on page 107); then
prepare a new run
The gel image looks
distorted
Incorrect Marker peaks
picked up
Depending on the
sample pattern wrong
peaks can get assigned
as marker if running
very close to the actual
marker
Use the Aligned button in the TapeStation
Analysis software to detect the correct markers.
Compare to aligned/unaligned data of good and
questionable samples; then assign correct
markers by right-clicking.
See the Agilent Information Center for more
information
Table 16 RNA - Possible causes of unexpected migration profile.
Problem Likely Cause Explanation Solution

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English