G4208A User’s Guide 109
3 Running an Isocratic Analysis
What You Will Need
What You Will Need
Instruments Agilent Series isocratic, binary or quaternary pump, an autosampler and a
UV- detector.
Column A 125 mm × 4.0 mm Hypersil ODS, 5 μm (Agilent Technologies part number
7982618- 564).
Solvents For the isocratic pump, a solvent mixture of LC grade bidistilled water (35 %)
and acetonitrile (65
%).
Sample The Agilent Technologies isocratic standard sample (Agilent Technologies
part number 01080- 68704). This contains 0.15 wt.% dimethylphthalate,
0.15 wt% diethylphthalate, 0.01 wt.% biphenyl and 0.03 wt.% o- terphenyl
dissolved in methanol.
The descriptions are based on Agilent 1100/1200 systems. The Agilent 1290
Infinity System may have other requirements on the setup and material.
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