G4208A User’s Guide 110
3 Running an Isocratic Analysis
Preparing the LC System
Preparing the LC System
1 For the isocratic pump, fill the solvent bottle with the mixture of LC- grade
bidistilled water (35
%) and acetonitrile (65 %). For the binary or quaternary
pump, fill one solvent bottle with bidistilled water (channel A) and the other
with acetonitrile (channel B).
2 Turn on the detector lamp and pump via the Control - System On/OFF screen.
3 For the quaternary pump, turn on the degasser by pressing the line-power
switch.
4 Purge the pump.
5 Allow the detector at least 15 min to provide a stable baseline.
6 Transfer the contents of an Agilent Technologies isocratic standard sample
ampoule into a vial and seal the vial with a cap. Place the vial in position 1 of
the autosampler tray.
7 Pump the water/acetonitrile (35/65 %) mobile phase through the column for
10
min at a flow rate of 2 mL/min.
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