G4208A User’s Guide 111
3 Running an Isocratic Analysis
Entering Settings
Entering Settings
To set up the isocratic analysis, you will set the LC system settings to default and
then modify selected settings. The other settings will remain with their default
values. You will then save these settings to a method called ISO.
1 Enter the Method screen.
2 Select Control and select System: Set Defaults.
3 Set the Stoptime to 6 min.
4 Set the Flow to 1.5 mL/min.
5 Set %B 65, (%C OFF, %D OFF for quaternary pump. If you have a binary pump,
set %B to 65.).
6 Set Injection volume to 1 µL.
The channels of a pumping system are named A, B, C and D (depending on the
pump type). %A is automatically calculated by 100 % - (%B + %C + %D). If no
values for %B, %C and %D are entered, %A is always 100 %.
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