3.
Select a well on the plate diagram to view the spectral results of the associated
capillary.
Status— Passed (dark green), Selected (light green), Borrowed/failed
(tan). If y
ou override a capillary, the status is set to
.
1
Well A01—Passed
2
Well B06—Selected
3
Well D04—Borrowed, failed, or overridden
4.
If necessary
, borrow calibration data from another capillary:
a.
Select a failed capillary.
a.
Click Override.
b.
Select a passing capillary from which to borrow the calibration.
5.
Evaluate the spectral calibration prole for each capillary. Ensure that the prole
meets the criteria listed in the following table.
See also“Magnify the spatial prole“ on page 96.
Attribute Acceptance criteria Example
Order of the peaks in
the spectral profile
(intensity vs pixel)
from left to right
4-dye: blue-green-
yellow-red
5-dye: blue-green-
y
ellow-red-orange
Chapter 8 Calibrate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
106
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
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