FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 85
Detail Graph
All the read modes for wells selected in the multi-well graph can be
displayed enlarged in a detail graph. You can select individual or
multiple wells to view, or select by group.
The data displayed in the Detail Graph have the same corrections
applied as the data displayed in the Multi-Well Graph. The Detail
Graph reports these settings at the top of the graph window:
• Subtract Bias
• Spatial Uniformity Correction
• Negative Control Correction
• Positive Control Scaling
• Response Over Baseline
• Crosstalk Correction
These correspond directly to options in the Correction dialog, opened
from the Analysis process page. To change these settings, change
them in the Correction dialog. This will change all data in the
Multi-Well Graph and Detail Graph to reflect the new correction
applied. Detail Graph allows a number of data-selection options:
Note: When the FLIPR
®
Tetra System is configured for two or more
read modes, it cycles through each mode alternating between them for
each reading. Although readings from each mode within a cycle occur
at different times, they are represented as occurring simultaneously.
Button Name Description
Copy
Graph
Copies Graph in the Detail Graph to the clipboard so it
can be pasted in a different program such as Microsoft
Word.
Copy
Graph
Data
Copies graph data in the Detail Graph to the clipboard
so it can be pasted in a different program such as
Microsoft Excel.
Zoom
Mode
Click to enable zoom capability in the graph.
Once the Zoom button is pressed, drag over a region in
the graph to view just that area enlarged to the full size
of the graph.
Alternatively, drag the cursor along a part of the x or y
axis to zoom in on just that axis.
Auto Scale Manual prompt to automatically scale the Detail Graph
and Multi-Well Graphs to include all data points of the
desired traces.
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