FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 93
Each of the correction options can be applied alone or in combination
with the others. When a combination is selected they are applied in the
following order:
1. Crosstalk
2. Spatial Uniformity Correction or Response Over Baseline
3. Negative Control Correction
4. Positive Control Scaling
5. Subtract Bias
Spatial
Uniformity
Correction
Check this option to use the initial signal to normalize signal
in each well, removing fluctuations due to well-to-well
variation in cell density and dye loading. The processing
algorithm averages the initial signal from all wells together.
It then scales each individual well relative to the average.
This correction is particularly recommended when you want
to normalize cell number, type and dye-loading conditions
throughout a plate.
Note: Spatial uniformity should only be used when
all wells in a plate are treated the same prior to an
experiment (for example, dye loading, cell numbers,
etc.).
Subtract Bias
Based On
Sample
Check this option to subtract the RLU measured at a
selected read number from all other time points in each
well. This option enables you to set the Y-axis scale so that
at the time point specified, the
Y-axis values for all data graphs is zero.
Select the read number to be subtracted in the field to the
right of the checkbox. The default is read number 1.
Response Over
Baseline
Check this option to display the trace as a ratio of the
response to the average of a set of predefined reads.
Baseline Start—First read to be included in the averaged
baseline value.
Baseline End—Last read to be included in the average
baseline value.
Show As Percentage—Displays response as a percent
increase over the average baseline value.
Item Description
Note: Spatial uniformity correction and percent baseline cannot be
applied at the same time.
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