FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 97
The statistics defined in the Group Statistic Table can be plotted in
the Group Statistic Graph, located behind the Detail Graph, to
quickly view a dose-response curve. All groups are included in the
graph, unless the user right-clicks on the desired group and selects
Exclude Groups From Statistic Chart. Groups not used in the graph
will have a green line through the entire row of the group. To include
the group in the graph, right-click the group and select Include
Groups In Statistic Chart. Data displayed in the Group Statistic
Graph is defined by selecting the desired group statistic from the
Group Statistic Table. Therefore, if the standard deviation is the
desired data to be evaluated, select the Standard Deviation column
header to highlight this data in the Group Statistic Table and it will be
displayed in the graph.
Select
Statistics
(continued
)
Z Score—Used to evaluate quality or performance of
the assay and is dependant on the concentration
evaluated
1
.
Z-factor—Used to evaluate the quality or
performance of the assay at a given concentration.
This is typically used for all concentrations, not
including the positive control. Calculation used:
Z = 1 - [3*(std dev of GROUP + std dev of neg
ctrls)/abs(mean of GROUP - mean of neg
ctrls)]
Z’-factor—A characteristic parameter for the
quality of the assay itself. This is typically
performed using data only from the positive control
concentration. Calculation used:
Z' = 1 - [3*(std dev of pos ctrls + std dev
of neg ctrls)/abs(mean of pos ctrls - mean of
neg ctrls)].
1. Zhang, J., Chung, T. D. Y. and Oldenburg, K. R. (1997). A Simple Statistical
Parameter for Use in Evaluation and Validation of High Throughput Screening
Assays. Journal of Biomolecular Screening 4(2):67:73.
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