Software Reference 123
P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
Analysis Settings Tab (Continued)
• Slope Threshold (rfu/sec) - Represents the amount of change
in absorbance units over time required to indicate that a peak
has occurred. This setting is used to detect the start and end of
a peak. Increasing this setting may cause broad rolling bumps to
be ignored or merge multiple bumps into a single peak.
Decreasing this setting will broaden the peaks’ width and
potentially pick up broad bumps as peaks.
• StartTime (sec) - Specifies the time after the start of a run when
the first peak will be detected (any peaks appearing before this
time are ignored). The Gel and Graph views will not plot data
earlier than this time.
• End Time (sec) - Specifies the time after which peak detection
stops. The Gel and Graph views will not plot data beyond this
time.
• Baseline start 1sec before - Specifies the time in seconds after
the start of the run when the first peak can appear. The Baseline
starts 1 second before this time.
• Exclude System Peaks check box - (Protein assays only) If
selected, system peaks are identified and excluded from
analysis.
• Ladder Ratio - (Protein assays only) Time at which ladder
peaks are detected as ladder peaks rather than system peaks.
For example, if the ladder ratio is set to 1.5, the software
multiplies the lower marker migration time x 1.5 and then begins
ladder peak identification.
• Minimum Sample Size - (Protein assays only) Specifies the
size at which peaks are identified as sample peaks rather than
system peaks (except the Lower Marker).
• Apply button - Applies the changes to the current run if data is
not being exported during the run.
• Cancel button - Cancels changes to the window and displays
the last saved values or the default values if no changes have
been saved.
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