Troubleshooting and Diagnostics 160
P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
Calibration Failures
Protein Clear HR and ProteinEXact assays undergo a Calibration
process at the onset of data collection. This section describes
possible causes and recommended actions for the calibration
failure for each assay:
• Protein Clear HR Assay Failed Calibration
• Protein Clear HR Assay Marginal Calibration
• ProteinEXact Assay Failed Calibration
Protein Clear HR Assay Failed Calibration
The Protein Clear HR Assay may display pop-up windows
indicating failed calibration outcomes. Failed calibration indicates
the assay is not able to collect data.
Possible Causes Recommended Actions
1. Poor ladder quality. Clean the electrode block per the instructions in the
Protein Clear HR assay user guide.
Re-prepare the chip, making sure the reagents are
fresh and the dye is completely thawed.
Reload the ladder well in the plate with fresh ladder
solution, and replace the VeriMAb sample in the plate
with fresh VeriMAb.
Retry calibration (see page 26).
If the problem is not resolved, contact PerkinElmer
Technical Support (see page 3).
2. Expected peaks for 120
kDa ladder protein or
standard sample proteins
cannot be identified.
3. Misidentification of
peaks.
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