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P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
Table of Contents
Preface ....................................................................................................................2
Instrument Safety ...................................................................................................8
Required Training................................................................................................8
Chemical Safety..................................................................................................9
Laser Safety......................................................................................................10
Electrical Safety ................................................................................................11
Mechanical Safety.............................................................................................13
Introduction...........................................................................................................18
Usage ...............................................................................................................18
Assay User and Quick Guides...........................................................................19
Principles of Operation......................................................................................20
Preparing the Instrument to Run Samples ..........................................................23
Opening the LabChip GX Touch Software .........................................................24
Purging the Pressure Lines ...............................................................................24
Inserting the Chip..............................................................................................25
Calibrating the Instrument to Run Protein Clear HR Assays ..............................26
Priming the Chip Before the Run .......................................................................27
Placing the Barcode on the Plate ......................................................................28
Defining a New Plate Type................................................................................29
Loading the Plate, Ladder Tube, and Buffer Tube .............................................31
Running an Assay.................................................................................................32
Defining the Run Parameters ............................................................................33
Monitoring the Run............................................................................................37
Editing the Analysis Settings during a Run ........................................................38
Stopping a Run before the Run is Complete......................................................39
Continuing a Stopped Run ................................................................................40
Removing the Plate, Buffer Tube, and Ladder Tube ..........................................41
Removing the Chip............................................................................................41
Washing the Chip..............................................................................................42
Exporting Data ......................................................................................................43
Export Examples ...............................................................................................44
Selecting the Auto Export Settings ....................................................................46
Selecting the Exported Properties in the Well Table or Peak Table ...................47
Exporting Data in AIA Format for Use in Third-Party Analysis Software.............47
Exporting a Data File after a Run is Complete...................................................48
Viewing the Data...................................................................................................49
Selecting the Well Data to View.........................................................................49
Viewing Data in the Egram/Gel Tab...................................................................50
Viewing Graphs in the Egram Tab.....................................................................52
Viewing Data in the Gel Tab..............................................................................52
Zoom In and Zoom Out .....................................................................................52
Viewing a Data File after a Run is Complete .....................................................53
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