Preparing the Instrument to Run Samples 26
P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
Calibrating the Instrument to Run Protein Clear
HR Assays
If a Protein Clear HR chip is inserted into the instrument, the user
must calibrate the instrument to run a Protein Clear HR assay
before starting the run. Calibration is performed by processing a
known protein sample, and uses the results to precisely set the
electrode currents for that assay to obtain consistent chip-to-chip
assay results.
To calibrate the instrument to run Protein Clear HR assays:
1 Insert the Protein Clear HR chip (see page 25) OR
Open the LabChip GX Touch software (see page 24) with the
chip already inserted into the instrument.
2 Touch the Prime and Calibrate button on the Home Window.
The Prime and Calibrate Window opens.
3 Select the plate type from the Select Plate Type drop-down list.
4 Select the assay from the Select Assay drop-down list. (To
select the location of the assay file, touch the Change Assay
Folder button and select the assay folder on the Change Assay
Folder Window.)
5 Touch the Select Well Containing Standard Sample button to
select the well that contains the VeriMAb
sample.
6 Select the desired well from the full-size plate diagram and
touch the Done button.
7 Touch the Prime and Calibrate button on the Prime and
Calibrate Window.
• If the calibration is successful, a dialog box opens indicating
that the instrument is now calibrated for the Protein Clear
HR assay. Touch the OK button.
• If the calibration fails, a dialog box opens indicating that the
calibration process failed and a Protein Clear HR assay run
cannot be performed. Touch the OK button. Perform the
recommended actions on page 160 and retry the calibration.
• If the calibration is outside the % Purity target range or if
dips from the Egrams could not be eliminated, a dialog box
opens indicating that the calibration is marginal. Touch the
Yes button to run the chip despite the issue displayed on the
dialog box. Touch No to fix the issue. Perform the
recommended actions on page 161 and retry the calibration.
8 To retry the calibration, select the Skip Prime Step check box
on the Prime and Calibrate Window. The chip is calibrated but
not primed.
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