PerkinElmer LabChip GXII Touch

Troubleshooting and Diagnostics 161
P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
Protein Clear HR Assay Marginal Calibration
Marginal calibration indicates data collection may proceed, but the
calibrated currents are outside the validated assay parameters. For
marginal calibration, collected data may be outside published assay
specifications, but qualitative electropherograms may be obtained.
Possible Causes Recommended Actions
1. Dips in EGram trace
could not be eliminated.
Touch the Yes button to run the chip despite the issue
displayed on the pop-up window.
OR
Touch the No button to fix the issues displayed on the
pop-up window. To fix the issues:
• Clean the electrode block per the instructions in the
Protein Clear HR assay user guide.
• Re-prepare the chip, making sure the reagents are
fresh and the dye is completely thawed.
• Reload the ladder well in the plate with fresh ladder
solution, and replace the VeriMAb sample in the
plate with fresh VeriMAb.
• Retry calibration (see page 26).
If the problem is not resolved, contact PerkinElmer
Technical Support (see page 3).
2. Could not achieve target
Percent Purity values.