2D-LC User Guide 113
5 2D-LC Data Acquisition in MassHunter Workstation 11
2D-LC User Interface
Transfer Volume
1
D detector
The
1
D settings for the transfer volumes that determine the time between the
1
D
detection of the peak and the switching of the 2D-LC valve, depend on the
hardware setup. For a standard 2D-LC with two DADs, the transfer volume is
approx. 14 µL. To calculate the volume, you have to add half the volume of the
detector flow cell plus the volume between the detector flow cell and the 2D-LC
valve.
Transfer Volume
2
D detector
The transfer volume possibility for the
2
D detector defines the volume between
the 2D-LC valve and the second dimension detector flow cell. This is the result
set for delay calculation in 2D-LC OpenLab DA-Plugin.
2
D offset between sample
loop and detector ensures, that start and end of a cut chromatogram are
aligned/displayed correctly.
If a second
1
D detector is installed, the transfer volume between the two
detectors in the first dimension volume must also be entered in the signal
selection of the reference chromatogram see “Method Parameters” on page 125.
For the MassHunter workflow with a High-End mass spectrometer as additional
second detector, you have to define this transfer volume (delay) during the file
splitting setup in the data evaluation.
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