2D-LC User Guide 151
6 Method Parameters
Set the 2D-LC Method parameters
• Down Slope
This method detects peaks based on down slope values only. The slope of
the falling peak triggers the peak cutting. The slope value is based on the
first derivative of the signal. The default value is 1.00 mAu/s.
• Upper Threshold
This method detects peaks based on upper threshold values only. The
height of the peak ensures that collection is not switched off, even for a
saturated signal that might be expected to do so. When the UV signal
exceeds the upper threshold, slope collection will be disabled. The default
value is 2000.000 mAu.
The 2D-LC Valve switches either:
• If the sampling time / cut size has elapsed (sampling time controls cut
position), or
• If the signal falls below the threshold or slope (peak-based)
whichever comes first
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